HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid

Herpes Simplex Virus by PCR, PCR for Herpes Simplex, UOW3537

The detection and quantitation of Herpes Simplex virus (HSV) by real-time PCR amplification. PCR detects amplicons of the gB gene for both HSV-1 and HSV-2. The two probes used in the reaction, specific either to HSV-1 or HSV-2 are labeled with different reporter dyes. Quantitation of HSV DNA (copies/mL) is achieved by amplifying a standard curve consisting of serial dilutions of a known amount of DNA containing the HSV-1 and HSV-2 amplicons. Results are reported in copies per milliliter (copies/mL). The minimum virus level that gives a positive result in 95% of replicates is 250 copies/mL (2.40 log copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration

Blood or Clear non-cellular fluid (e.g. BAL, Washing).

Blood: 3 mL blood in EDTA (LAVENDER TOP) tube.

Eye Fluid: 50 microliters in sterile container.

NOTE: Do not send syringe with needle. Only send syringe or transfer fluid to sterile container.

Clear non-cellular fluid (e.g. BAL or Washing): 1 mL sterile container.

Also Acceptable: 3 mL blood in GOLD SST, RED, or PPT (PEARL TOP) tube.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum sample requirement: 0.5 mL

Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder. Do not freeze/thaw.

Eye Fluid: Freeze at -10°C or colder. Do not freeze/thaw.

Clear non-cellular fluid: Freeze at -10°C or colder. Do not freeze/thaw.

0.5 mL serum/plasma/non cellular fluid.

Frozen

Virology, Eastlake (EVIR)

Real-time Polymerase Chain Reaction.

Tuesday and Friday

Code                                   Name
HSVQNS                          HSV Specimen_PCR Quant
HSVQN1                          HSV Type 1 Quant PCR Result
HSVLO1                            HSV1 DNA Copies/mL (Log 10)
HSVQN2                           HSV Type 2 Quant PCR Result
HSVLO2                            HSV2 DNA Copies/mL (Log 10)
HSVQNI                             HSV Interpretation_PCR Quant
HSVQNM                          HSV Type 1 and 2 Quant PCR Method

87530x2

No

UW

49381-7