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Flow Cytometry

 

Specimen Requirements and Handling

Test Sample Method

Flow Cytometry

FISH

Cytogenetics 

Peripheral Blood
  • One full 5 mL (lavender top) potassium EDTA tube is preferred, or one full 5 mL (green top) sodium heparin tube is acceptable.
  • Send to lab as soon as possible at room temperature
Bone Marrow
  • 3-5 mL bone marrow immediately placed into a sodium heparin (green top) or potassium EDTA (lavender top) tube. 
  • In case of "dry tap" a piece of bone marrow core may be submitted according to the guidelines below for solid tissue submission.
  • Send to lab as soon as possible at room temperature
Solid Tissue (including lymph nodes)
  • Please submit as much tissue as possible, avoiding any necrotic foci.
  • Tissue should be cut into small 2-3 millimeter pieces and placed in RPMI preparation provided by laboratory.
  • Sterile saline may be used if no RPMI is available. 
  • AVOID FIXATIVES. 
  • Send to lab as soon as possible at room temperature
Cerebrospinal Fluid

     To fresh CSF:

  • Add 1-2ml RPMI enrichment media (available upon request)
  • Deliver to lab immediately
  • Provide copy of cell count/differential results
  • Recovery of viable cells is dependent of cellularity of original specimen and timely processing
PCR Peripheral Blood
  • EDTA (lavender top) whole blood 14 mL (2 tubes) preferred
  • RNA based PCR test requires a very fresh sample because RNA degrades much faster than DNA - send to lab within 48 hours
Bone Marrow
  • 0.5 cc marrow in an EDTA (lavender top) tube
  • RNA based PCR test requires a very fresh sample because RNA degrades much faster than DNA - send to lab within 48 hours